Journal
JOURNAL OF VIROLOGY
Volume 74, Issue 3, Pages 1234-1240Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/JVI.74.3.1234-1240.2000
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Funding
- NCI NIH HHS [CA50777] Funding Source: Medline
- NIAID NIH HHS [5T32 AI07403, AI34834, T32 AI007403] Funding Source: Medline
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The diploid nature of human immunodeficiency virus type 1. (HIV-1) indicates that recombination serves a central function in virus replication and evolution. Previously, while examining the nature of obligatory primer strand transfers during reverse transcription, a high rate of recombination was observed at the ends of the viral genome within the viral long terminal repeats, prompting the following question: does recombination occur at a high rate throughout the genome? To address this question, two vectors based upon different strains of HIV-1 were utilized. The vectors were comprised predominantly of autologous HIV-1 sequence and were approximately the same size as the parental genome. The proviral progeny of heterodimeric virions were analyzed after a single cycle of replication, and the sequence heterogeneity between the two strains allowed direct examination of recombination crossovers. The results obtained indicate that HIV-1 undergoes approximately two to three recombination events per genome per replication cycle. These results imply that both HIV-1 RNAs are typically utilized during reverse transcription and that recombination is an important aspect of HIV-1 replication.
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