Journal
JOURNAL OF CELL SCIENCE
Volume 128, Issue 15, Pages 2919-2927Publisher
COMPANY OF BIOLOGISTS LTD
DOI: 10.1242/jcs.172072
Keywords
AINTEGUMENTA (ANT); SOS3-LIKE CALCIUM BINDING PROTEIN8; SCABP8; CALCINEURIN B-LIKE10; Salt stress; Trans-repression; Shoot; Arabidopsis
Categories
Funding
- National Key Basic Research Program of China [2014CB954100]
- National Key Technology RD Program [2015BAD15B02]
- Tianshui Normal University
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The Arabidopsis AINTEGUMENTA (ANT) gene, which encodes an APETALA2 (AP2)-like transcription factor, controls plant organ cell number and organ size throughout shoot development. ANT is thus a key factor in the development of plant shoots. Here, we have found that ANT plays an essential role in conferring salt tolerance in Arabidopsis. ant-knockout mutants presented a salt-tolerant phenotype, whereas transgenic plants expressing ANT under the 35S promoter (35S:ANT) exhibited more sensitive phenotypes under high salt stress. Further analysis indicated that ANT functions mainly in the shoot response to salt toxicity. Target gene analysis revealed that ANT bound to the promoter of SOS3-LIKE CALCIUM BINDING PROTEIN 8 (SCABP8), which encodes a putative Ca2+ sensor, thereby inhibiting expression of SCABP8 (also known as CBL10). It has been reported that the salt sensitivity of scabp8 is more prominent in shoot tissues. Genetic experiments indicated that the mutation of SCABP8 suppresses the ant-knockout salt-tolerant phenotype, implying that ANT functions as a negative transcriptional regulator of SCABP8 upon salt stress. Taken together, the above results reveal that ANT is a novel regulator of salt stress and that ANT binds to the SCABP8 promoter, mediating salt tolerance.
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