Journal
ENDOCRINOLOGY
Volume 141, Issue 2, Pages 560-563Publisher
ENDOCRINE SOC
DOI: 10.1210/en.141.2.560
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The role of glucocorticoids in obesity is poorly understood. Observations in obese men suggest enhanced inactivation of cortisol by 5 alpha-reductase and altered reactivation of cortisone to cortisol by 11 beta-hydroxysteroid dehydrogenase type 1 (11 beta HSD1). These changes in glucocorticoid metabolism may influence corticosteroid receptor activation and feedback regulation of the hypothalamic-pituitary-adrenal axis (HPA). We have compared corticosterone metabolism in vivo and in vitro in male obese and lean Zucker rats, aged 9 weeks (n = 8/group). Steroids were measured in 72-h urine and 0900 h trunk blood samples. 5 alpha-Reductase type 1 and 11 beta HSD activities were assessed in dissected tissues. Obese animals were hypercorticosteronemic and excreted more total corticosterone metabolites (2264 +/- 623 us. 388 +/- 144 ng/72 h; P = 0.003), with a greater proportion being 5 alpha-reduced or 11-oxidized. 11-Dehydrocorticosterone was also elevated in plasma (73 +/- 9 us. 18 +/- 2 nM; P = 0.001) and urine (408 +/- 111 us. <28 ng/72 h; P = 0.01). In liver of obese rats, 5 alpha-reductase type 1 activity was greater (20.6 +/- 2.7% vs. 14.1 +/- 1.5%; P < 0.04), but 11 beta HSD1 activity (maximum velocity, 3.43 +/- 0.56 vs. 6.57 +/- 1.13 nmol/min/mg protein; P = 0.01) and messenger RNA levels (0.56 +/- 0.08 vs. 1.03 +/- 0.15; P = 0.02) were lower. In contrast, in obese rats, 11 beta HSD 1 activity was not different in skeletal muscle and sc fat and was higher in omental fat (36.4 +/- 6.2 vs. 19.2 +/- 6.6; P = 0.01), whereas 11 beta HSD2 activity was higher in kidney (16.7 +/- 0.6% us. 11.3 +/- 1.5%; P = 0.01). We conclude that greater inactivation of glucocorticoids by 5 alpha-reductase in liver and 11 beta HSD2 in kidney combined with impaired reactivation of glucocorticoids by 11 beta HSD1 in liver may increase the MCR of glucocorticoids and decrease local glucocorticoid concentrations at these sites. By contrast, enhanced 11 beta HSD1 in omental adipose tissue may increase local glucocorticoid receptor activation and promote obesity.
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