Journal
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
Volume 66, Issue 2, Pages 684-687Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/AEM.66.2.684-687.2000
Keywords
-
Categories
Ask authors/readers for more resources
The conversions of vanillic acid and O-benzylvanillic acid to vanillin were examined by using whole cells and enzyme preparations of Nocardia sp. strain NRRL 5646. With growing cultures, vanillic acid was decarboxylated (69% yield) to guaiacol and reduced (11% yield) to vanillyl alcohol. In resting Nocardia cells in buffer, 4-O-benzylvanillic acid was converted to the corresponding alcohol product without decarboxylation. Purified Nocardia carboxylic acid reductase, an ATP and NADPH-dependent enzyme, quantitatively reduced vanillic acid to vanillin. Structures of metabolites were established by H-1 nuclear magnetic resonance and mass spectral analyses.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available