Journal
ANESTHESIOLOGY
Volume 92, Issue 2, Pages 485-492Publisher
LIPPINCOTT WILLIAMS & WILKINS
DOI: 10.1097/00000542-200002000-00031
Keywords
antinociception; blind patch; clamp recording; intracellular recording; IPSC
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Background: It has been reported previously that norepinephrine, when applied to the spinal cord dorsal horn, excites a subpopulation of dorsal horn neurons, presumably inhibitory interneurons, in the current study, the authors tested whether norepinephrine could activate inhibitory interneurons, specifically those that are ''GABAergic.'' Methods: A transverse slice was obtained from a segment of the lumbar spinal cord isolated from adult male Sprague-Dawley rats. Whole-cell patch-clamp recordings were made from substantia gelatinosa neurons using the blind patch-clamp technique. The effects of norepinephrine on spontaneous GABAergic inhibitory postsynaptic currents were studied. Results: In the majority of substantia gelatinosa neurons tested, norepinephrine (10-60 mu M) significantly increased both the frequency and the amplitude of GABAergic inhibitory postsynaptic currents, These increases were blocked by tetrodotoxin (1 mu M). The effects of norepinephrine mere mimicked by the alpha(1)-receptor agonist phenylephrine (10-80 mu M) and inhibited by the alpha(1)-receptor antagonist WB-4101 (0.5 mu M). Primary-afferent-evoked polysynaptic excitatory postsynaptic potentials or excitatory postsynaptic currents in wide-dynamic-range neurons of the deep dorsal horn were also attenuated by phenylephrine (40 mu M). Conclusion: The observations suggest that GABAergic interneurons possess somatodendritic alpha(1) receptors, and activation of these receptors excites inhibitory interneurons, The alpha(1) actions reported herein may contribute to the analgesic action of intrathecally administered phenylephrine.
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