Journal
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
Volume 89, Issue 2, Pages 199-202Publisher
SOC BIOSCIENCE BIOENGINEERING JAPAN
DOI: 10.1016/S1389-1723(00)88738-1
Keywords
Corynebacterium sp.; alginate lyase; poly(alpha-L-guluronate)lyase; cloning
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The aly PG gene, coding for a poly alpha-L-guluronate lyase (PG lysase) of Corynebacterium strain ALY-1, was cloned and sequenced. The gene consists of 768 bp encoding a signal peptide of 32 amino acids and a mature protein of 224 amino acids. Two disulfide bond cross-linkages were found to be formed between Cys-4 and Cys-51 and between Cys-200 and Cys-206 in the native PG lyase molecule. The deduced amino acid sequence of the Corynebacterium sp. aly PG gene exhibited 29% homology toward that of the Klebsiella pneumoniae, subsp. aerogenes aly A gene, with two conserved regions (the amino acid sequences from Y-102 to M-110 and from Y-221 to Q-229).
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