Journal
JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY
Volume 48, Issue 2, Pages 303-311Publisher
HISTOCHEMICAL SOC INC
DOI: 10.1177/002215540004800216
Keywords
bombesin; cancer; gastrin-releasing peptide; methods
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Funding
- NIDDK NIH HHS [DK51168] Funding Source: Medline
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Currently available techniques for performing quantitative immunohistochemistry (Q-IHC) rely upon pixel-counting algorithms and therefore cannot provide information as to the absolute amount of chromogen present. We describe a novel algorithm for true Q-IHC based on calculating the cumulative signal strength, or energy, of the digital file representing any portion of an image. This algorithm involves subtracting the energy of the digital file encoding the control image (i.e., not exposed to antibody) from that of the experimental image (i.e., antibody-treated). In this manner, the absolute amount of antibody-specific chromogen per pixel can be determined for any cellular region or structure.
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