Journal
JOURNAL OF BACTERIOLOGY
Volume 182, Issue 4, Pages 1172-1175Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/JB.182.4.1172-1175.2000
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Funding
- NIGMS NIH HHS [F32 GM020478, GM 20478, R01 GM020478, R37 GM020478] Funding Source: Medline
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Cardiolipin (CL)-specific fluorescent dye 10-N-nonyl-acridine orange (NAO) was used to visualize CL distribution in Escherichia coli cells of different phospholipid compositions. In a filamentous mutant containing only anionic phospholipids, green fluorescent spots were observed along the filaments at approximately regular intervals. Three-dimensional image reconstruction obtained by optical sectioning and a deconvolution algorithm revealed NAO-binding domains in the plane of the cell membrane. Substantial red fluorescence emission of bound NAO supported labeling of CL-containing domains. These structures were not found in mutants deficient in CL biosynthesis. The domains were also observed mostly in the septal region and on the poles in cells of normal size with wild-type phospholipid composition.
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