Journal
BIOMEDICINE & PHARMACOTHERAPY
Volume 62, Issue 9, Pages 659-666Publisher
ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER
DOI: 10.1016/j.biopha.2008.08.002
Keywords
Paeoniflorin; NF-kappa B; I kappa B alpha; Apoptosis; Gastric carcinoma cells
Funding
- principal project of science and technology of Jiangsu Province [BS2007011]
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Objective: We sought to determine whether paeoniflorin enhances 5-fluorouracil-induced apoptosis in human gastric carcinoma cells, and, if so, to determine the relationship between this apoptosis and NF-kappa B activation. Methods: Paeoniflorin was diluted to different concentrations and added to gastric carcinoma cells (SGC-7901) at different times. Western blot was used to test the expression of NF-kappa B in nuclear and I kappa B alpha, p-I kappa B alpha, IKK alpha in cytoplasm. Further, the intranuclear expression of NF-kappa B was confirmed by ELISA assay. The impact of paeoniflorin and 5-fluorouracil on cell apoptosis of gastric carcinoma cells was estimated by flow cytometry. Results: Paeoniflorin revealed dramatic inhibition of NF-kappa B activity in the nuclei of the cells. The inhibition pattern of NF-kappa B was exhibited in a time- and dose-dependent manner, which was confirmed by Western blot and ELISA. Decreased nuclear translocation of IF-kappa B induced by paeoniflorin was found by preventing I kappa B alpha phosphorylation. Moreover. 5-fluorouracil-induced cell apoptosis was promoted by paeoniflorin in gastric carcinoma cells. Conclusions: Paeoniflorin can inhibit NF-kappa B activity of SGC-7901 cells. and enhance 5-fluorouracil-induced apoptosis of gastric carcinoma cells. (C) 2008 Elsevier Masson SAS. All rights reserved.
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