Journal
EMBO JOURNAL
Volume 19, Issue 3, Pages 410-420Publisher
WILEY
DOI: 10.1093/emboj/19.3.410
Keywords
Mex67p; nuclear RNA export; nucleocytoplasmic transport; RNA chaperone; Yra1p
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Mex67p and Mtr2p constitute an essential mRNA export complex that interacts with poly(A)(+) RNA and nuclear pore proteins, We have identified Yra1p, an intranuclear protein with in vitro RNA-RNA annealing activity, which directly binds to Mex67p. The complex between Yra1p and Mex67p was reconstituted in vitro and shown by UV-crosslinking to bind directly to RNA. Mutants of YRA1 are impaired in nuclear poly(A)(+) RNA export at restrictive growth conditions. ALY, the mouse homologue of Yra1p and a transcriptional coactivator, can bind in vitro to yeast and human Mex67p and partly complements the otherwise non-viable yra1 null mutant. Thus, Yra1p is the first RNA-binding protein characterized, which bridges the shuttling Mex67p/Mtr2p exporter to intranuclear mRNA transport cargoes.
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