Journal
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
Volume 66, Issue 2, Pages 836-838Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/AEM.66.2.836-838.2000
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The penetration of hydrogen peroxide into biofilms formed by wild-type and catalase-deficient Pseudomonas aeruginosa strains was measured using microelectrodes. A flowing stream of hydrogen peroxide (50 mM, 1 h) was unable to penetrate or kill wild-type biofilms but did penetrate and partially kill biofilms formed by an isogenic strain in which the katA gene was knocked out. Catalase protects aggregated bacteria by preventing full penetration of hydrogen peroxide into the biofilm.
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