Journal
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
Volume 89, Issue 2, Pages 212-214Publisher
SOC BIOSCIENCE BIOENGINEERING JAPAN
DOI: 10.1016/S1389-1723(00)88742-3
Keywords
sucrose permease; symport; kinetics; Saccharomyces
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The kinetic analysis of active sucrose-H+ uptake by Saccharomyces cerevisiae revealed the presence of two transport systems with high and low affinity for sucrose. The MAL2T permease has a low affinity (K-m=120+/-20 mM) for sucrose, while the alpha-glucoside transporter encoded by the AGT1 gene is a high affinity sucrose-H+ symporter (K-m=7.9+/-0.8 mM) that increases the specific growth rate of cells growing on sucrose.
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