Journal
JOURNAL OF MAGNETIC RESONANCE
Volume 142, Issue 2, Pages 276-279Publisher
ACADEMIC PRESS INC
DOI: 10.1006/jmre.1999.1954
Keywords
chemical exchange; heteronuclear 3D NMR; AlcR; protein; complex
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The potentialities of a 2D proton-detected heteronuclear exchange experiment to assign the nitrogen and amide proton resonances in a uniformly N-15-enriched macromolecule involved in a complex, starting from the free form assignments, are demonstrated on a protein-DNA complex. This 2D experiment is further extended to a 3D experiment in the case of severe superpositions. (C) 2000 Academic Press.
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