Journal
PLANTA MEDICA
Volume 66, Issue 1, Pages 57-62Publisher
GEORG THIEME VERLAG
DOI: 10.1055/s-2000-11115
Keywords
Artemisia annua L. (Asteraceae); artemisinin; artemisinic acid reductase; biosynthetic pathway; dihydroartemisinic acid; chemotypes
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The time course of the levels of artemisinin, its biosynthetic precursors and the biosynthetically related sesquiterpenes was monitored during a vegetation period of Artemisia annua plants of different geographical origin. Considerable differences in contents of artemisinin and its direct precursors artemisinic acid and dihydroartemisinic acid were found between these A. annua's, For the first time the A. annua plants of different geographical origin were found to belong to different chemotypes. A chemotype with a high artemisinin level was found to have also a high dihydroartemisinic acid level but a relatively low artemisinic acid level. Reversibly, a chemotype with low levels of artemisinin and dihydroartemisinic acid contained a high artemisinic acid level. Artemisinic acid is considered to be the direct precursor of dihydroartemisinic acid in the biosynthetic pathway of artemisinin. The observed accumulation of artemisinic acid in one of the A. annua chemotypes may indicate the presence of a rate-limiting step in the biosynthetic pathway of artemisinin. The enzymatic reduction of artemisinic acid into dihydroartemisinic acid is probably a bottle neck in the biosynthetic pathway of artemisinin in varieties with high artemisinic acid and consequentially low artemisinin levels, after a night-frost period, the level of artemisinin was increased, in the Vietnamese A. annua plants, while the dihydroartemisinic acid level was decreased, This phenomenon is in accordance with our hypothesis that stress triggers the conversion of dihydroartemisinic acid to artemisinin. It is suggested that the presence of high levels of dihydroartemisinic acid may be an adaptation to stress conditions (e.g., night-frost), during which relatively high levels of O-1(2) are formed. Dihydroartemisinic acid gives the plant protection by reading with these reactive oxygen species yielding artemisinin as stable end-product.
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