Journal
EXPERIMENTAL CELL RESEARCH
Volume 254, Issue 2, Pages 257-268Publisher
ELSEVIER INC
DOI: 10.1006/excr.1999.4771
Keywords
programmed cell death; cell-cell communication; connexin 43; bystander effect; stem cells; cancer; epithelial
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Funding
- NCI NIH HHS [CA21104] Funding Source: Medline
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The sequence of events involved in maintenance of homeostasis must encompass mechanisms within single cells as well as interactions between cells within a population. To investigate the interaction among these inter- and intracellular mechanisms, disruption of homeostasis by serum deprivation was performed in WB-F344, a normal diploid epithelial cell line. Changes in cell-cell communication (gap junction function) at the population level and in individual cells were monitored using the scrape load/dye transfer and fluorescence redistribution after photobleaching assays. Apoptosis and mitosis were measured using internucleosomal DNA ladder assays and fluorescence-activated cell sorting. The results indicate that a common element in early apoptosis and early mitosis is sustained gap junction function. As cell life (mitosis) and cell death (apoptosis) progressed, a common process of change in gap junction function occurred. A transient stimulation of mitosis concomitant with increased apoptosis was also observed during serum deprivation. Gap junctions may play a regulatory role during initiation of these opposite yet equally important mechanisms of maintaining homeostasis, This model system is useful for further studies on the relationships among inter- and intracellular mechanisms of homeostasis. (C) 2000 Academic Press.
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