Journal
BIOMEDICAL MICRODEVICES
Volume 16, Issue 5, Pages 737-743Publisher
SPRINGER
DOI: 10.1007/s10544-014-9878-9
Keywords
ELISA; Serological survey; Microfluidic device; West Nile viral antibodies; microplate reader
Funding
- National Science Foundation (DBI) [0964211]
- Wyoming INBRE [P20RR016474, P20GM103432]
- Direct For Biological Sciences
- Div Of Biological Infrastructure [0964211] Funding Source: National Science Foundation
Ask authors/readers for more resources
Serological surveys are vital to determining the prevalence of a disease and/or the immunity status against it in any population. However, the relatively large sample volume requirement (1-10 mL) in traditional serum-based assays demands that blood draw camps be set up by medical professionals to obtain samples for these studies which significantly increases the time and cost associated with them. Here we address these drawbacks of a serosurvey by reducing the whole blood requirement in its diagnostic procedures down to 10 mu L using the microfluidic platform. Such a miniaturization approach was demonstrated in our current work by developing a microchip based serological device for determining the serum levels of West Nile (WN) viral antibodies (IgG and IgM) to assess the immunity status against WN virus in Fremont County, Wyoming. Enzyme-linked immunosorbent assays (ELISA) were developed for these target analytes in glass microchannels to accomplish this task using antibodies/assay reagents purchased from commercial sources. The reported assays were directly quantitated using a fluorescence microplate reader which to our knowledge is the first account of signal measurement in a microchip based ELISA procedure using this standard instrument. To enable this quantitation method, the assay channels on our device were spaced identically as the wells on a commercial microplate, and a holder having the dimensions of this plate was used to accommodate the microchips. Our microfluidic assays showed an excellent correlation with the results from the microwell plate based experiments for significantly lower incubation periods and using only 3 mu L of the ELISA reagents.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available