Journal
BIOMEDICAL MICRODEVICES
Volume 15, Issue 1, Pages 135-143Publisher
SPRINGER
DOI: 10.1007/s10544-012-9695-y
Keywords
Microelectrode arrays; Impedance spectroscopy; Mitochondrial membrane potential; Oxygen glucose deprivation; Insulin-like growth factor 1; Drug screening
Funding
- National Science Council of Taiwan [NSC 98-2627-B-006-019, NSC 99-2627-B-006-018]
- National Health Research Institutes of Taiwan [NHRI-EX-98-9835EI]
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Microelectrode arrays (MEAs) incorporated with the electric cell substrate impedance sensing (ECIS) technique provide a method for acquiring cellular electrophysiological information, which is useful for the time-course monitoring of cellular outgrowth and damage. This research utilizes the ECIS technique for monitoring the time-course impedimetric changes in normal and insulin-like growth factor 1 (IGF-1)-protected cortical neurons under the ischemic insult of oxygen glucose deprivation (OGD) created in a microperfusion environment. The neuronal apoptosis is reflected by the relatively low cell viability (28 +/- 11.5 %) after 30-min OGD followed by 24 h of re-oxygenation. Also the hyperpolarization phase of mitochondrial membrane potential (MMP) occurs during 2 h of the re-oxygenation period. In contrast, cortical neurons treated with 50 and 100 ng/mL IGF-1 show higher survival rates of 45 +/- 5.2 % and 49 +/- 9.2 %, respectively, and no occurrence of the hyperpolarization of MMP during the re-oxygenation period. The ECIS results demonstrate that the measured impedance of cortical neurons decreased from 826 +/- 86 k Omega to 224 +/- 32 k Omega due to cell detachment under the insult of OGD. The measured impedance of IGF-1-protected cortical neurons slowly decreased to about 50 % of the original value (560 +/- 45 k Omega for 50 ng/mL and 593 +/- 44 k Omega for 100 ng/mL) compared to saline control of 232 +/- 37 k Omega, which indicates improved cell adhesion under OGD conditions. The time-course impedimetric results show that the proposed ECIS-based MEAs platform incorporated with a microperfusion environment can be used for the real-time monitoring of cortical neurons under in vitro OGD and the IGF-1 protective effect against OGD-induced ischemic neuronal death.
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