Journal
BIOMEDICAL MICRODEVICES
Volume 15, Issue 4, Pages 635-643Publisher
SPRINGER
DOI: 10.1007/s10544-012-9721-0
Keywords
Inhibition of cancer; Microfluidics; Tapered channels; Aggressive migration
Funding
- NSF CAREER [ECCS-0845669]
- NIH [GM095280, 1-R21-HG00576301]
- Welch Foundation [F-1654]
- NCI [5R01CA119388-05]
- NATIONAL CANCER INSTITUTE [R01CA119388] Funding Source: NIH RePORTER
- NATIONAL HUMAN GENOME RESEARCH INSTITUTE [R21HG005763] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [R01GM094933, F32GM095280] Funding Source: NIH RePORTER
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Tumor cells depict two deviant tendencies; over-proliferation and vigorous migration. A tapered channel device is designed and fabricated for in vitro studies. We report inhibited proliferation and migration of human glioblastoma (hGBM) cells when exposed to an aptamer that is known to bind epidermal growth factor receptors (EGFR). The device is integrated with controlled ambient and microscope for providing real-time and quantitative characterization of the tumor cell behavior. The results show that hGBM cells loose proliferation and motility when exposed to the anti-EGFR aptamer. The aptamer directly inhibits and blocks EGF-induced EGFR phosphorylation. This also reduces the ability of cells to remodel their internal structure for invasion through narrow constrictions. This provides a framework for possible studies on efficacy of other inhibiting molecules.
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