Journal
BIOMEDICAL MICRODEVICES
Volume 13, Issue 4, Pages 753-758Publisher
SPRINGER
DOI: 10.1007/s10544-011-9545-3
Keywords
Microfluidics; 3D cell culture; Mammary epithelial cells; MCF-10A
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The ability to culture cells in three dimensional extracellular matrix (3D ECM) has proven to be an important tool for laboratory biology. Here, we demonstrate a microfluidic perfusion array on a 96-well plate format capable of long term 3D ECM culture within biomimetic microchambers. The array consists of 32 independent flow units, each with a 4 mu l open-top culture chamber, and 350 mu l inlet and outlet wells. Perfusion is generated using gravity and surface tension forces, allowing the array to be operated without any external pumps. MCF-10A mammary epithelial cells cultured in Matrigel in the microfluidic array exhibit acinus morphology over 9 days consistent with previous literature. We further demonstrated the application of the microfluidic array for in vitro anti-cancer drug screening.
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