Journal
BIOMEDICAL MICRODEVICES
Volume 12, Issue 3, Pages 457-464Publisher
SPRINGER
DOI: 10.1007/s10544-010-9402-9
Keywords
Silica nanoparticles; Elastomeric membranes; Protein microarray; Three-dimensional template; Micropatterning
Funding
- MEST [R11-2007-05003002-0, M10755020001-08N5502-00110]
- National Research Foundation of Korea [2007-2004045] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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We used an assembly of silica nanoparticles (SNPs) as a three-dimensional template for protein immobilization to prepare a protein microarray with enhanced protein loading capacity and detection sensitivity. SNPs were first modified with 3-aminopropyltriethoxysilane (APTES) for covalent immobilization of protein and micropatterned on poly(ethylene glycol)(PEG)-coated glass slides using elastomeric membranes with an array of holes. Proteins were selectively immobilized only on the SNP region, while the PEG regions served as an effective barrier to protein adsorption. Because of multi-layered SNPs that had curved surface, protein loading in the SNP micropattern was about six times greater than on a planar surface, as observed by fluorescence microscopy, which consequently improved the protein activity and reaction rate. GOX-catalyzed glucose oxidation and the molecular recognition mediated, specific binding between biotin and streptavidin were both successfully assayed using SNP microarrays, with better fluorescence signal and sensitivity than corresponding planar microarrays.
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