Journal
JOURNAL OF CELL BIOLOGY
Volume 211, Issue 6, Pages 1141-1156Publisher
ROCKEFELLER UNIV PRESS
DOI: 10.1083/jcb.201508072
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Funding
- Wellcome Trust [107022, 103139]
- [077707]
- [092076]
- [091020]
- Grants-in-Aid for Scientific Research [15H01477, 26440190] Funding Source: KAKEN
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Kinetochores orchestrate mitotic chromosome segregation. Here, we use quantitative mass spectrometry of mitotic chromosomes isolated from a comprehensive set of chicken DT40 mutants to examine the dependencies of 93 confirmed and putative kinetochore proteins for stable association with chromosomes. Clustering and network analysis reveal both known and unexpected aspects of coordinated behavior for members of kinetochore protein complexes. Surprisingly, CENP-T depends on CENP-N for chromosome localization. The Ndc80 complex exhibits robust correlations with all other complexes in a core kinetochore network. Ndc80 associated with CENP-T interacts with a cohort of Rod, zw10, and zwilch (RZZ) interacting proteins that includes Spindly, Mad 1, and CENP-E. This complex may coordinate microtubule binding with checkpoint signaling. Ndc80 associated with CENP-C forms the KMN (Knl1, Mis12, Ndc80) network and may be the microtubule-binding workhorse of the kinetochore. Our data also suggest that CENP-O and CENP-R may regulate the size of the inner kinetochore without influencing the assembly of the outer kinetochore.
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