Journal
BIOMEDICAL MICRODEVICES
Volume 12, Issue 1, Pages 145-151Publisher
SPRINGER
DOI: 10.1007/s10544-009-9369-6
Keywords
Microfluidics; Alginate; Hydrogel; Endothelial cells; Osteoblastic cells; Caged calcium
Funding
- U.S. Army Research Laboratory
- U.S. Army Research Office [DAAD19-03-1-0168]
- NIH [HG004653]
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This paper describes a simple reversible hydrogel patterning method for 3D cell culture. Alginate gel is formed in select regions of a microfluidic device through light-triggered release of caged calcium. In the pre-gelled alginate solution, calcium is chelated by DM-nitrophen (DM-n) to prevent cross-linking of alginate. After sufficient UV exposure the caged calcium is released from DM-n causing alginate to cross-link. The effect of using different concentrations of calcium and chelating agents as well as the duration of UV exposure is described. Since the cross-linking is based on calcium concentration, the cross-linked alginate can easily be dissolved by EDTA. We also demonstrate application of this capability to patterned microscale 3D co-culture using endothelial cells and osteoblastic cells in a microchannel.
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