Journal
SCIENCE
Volume 287, Issue 5455, Pages 1034-1036Publisher
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.287.5455.1034
Keywords
-
Categories
Funding
- NIGMS NIH HHS [GM-28007] Funding Source: Medline
Ask authors/readers for more resources
Gradients of chemoattractants elicit signaling events at the Leading edge of a cell even though chemoattractant receptors are uniformly distributed on the cell surface. In highly polarized Dictyostelium discoideum amoebas, membrane-associated beta gamma subunits of heterotrimeric guanine nucleotide-binding proteins (G proteins) were localized in a shallow anterior-posterior gradient. A uniformly applied chemoattractant generated binding sites for pleckstrin homology (PH) domains on the inner surface of the membrane in a pattern similar to that of the G beta gamma subunits. Loss of cell polarity resulted in uniform distribution of both the G beta gamma subunits and the sensitivity of PH domain recruitment. There observations indicate that G beta gamma subunits are not sufficiently localized to restrict signaling events to the Leading edge but that their distribution may determine the relative chemotactic sensitivity of polarized cells.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available