Journal
BIOMEDICAL MICRODEVICES
Volume 11, Issue 2, Pages 323-329Publisher
SPRINGER
DOI: 10.1007/s10544-008-9238-8
Keywords
Microfluidics; Leukocyte; Aqueous two phase separation; Blood; PEG; Dex
Funding
- NIH [U54 CA119343]
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Leukocytes from a whole blood sample were concentrated using a microfluidic aqueous two phase system (mu ATPS). Whole blood was simultaneously exposed to polyethylene glycol (PEG) and dextran (Dex) phase streams and cells were partitioned based on their differential affinity for the streams. The laminar flow characteristic of microfluidic devices was used to create zero, one, and two stable interfaces between the polymer streams. Three different patterns of three polymer streams each were evaluated for their effectiveness in concentrating leukocytes: immiscible PEG-PEG-Dex, immiscible Dex-PEG-Dex, and miscible PEG-PBS-Dex. The most effective configuration was the Dex-PEG-Dex stream pattern which on average increased the ratio of leukocytes to erythrocytes by a factor of 9.13 over unconcentrated blood.
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