Journal
BIOMEDICAL MATERIALS
Volume 9, Issue 4, Pages -Publisher
IOP PUBLISHING LTD
DOI: 10.1088/1748-6041/9/4/045008
Keywords
alginate hydrogel; copper (II) ions; antibacterial activity; blood coagulation; wound dressing
Funding
- Thailand Research Fund (TRF) [DBG5280015]
- Integrated Innovation Academic Center IIAC Chulalongkorn University Centenary Academic Development Project, Chulalongkorn University [RES_01_54_63]
- Center of Excellence on Petrochemical and Materials Technology, Chulalongkorn University
- The Petroleum and Petrochemical College (PPC), Chulalongkorn University, Thailand
Ask authors/readers for more resources
The incorporation of a metal ion, with antimicrobial activity, into an alginate dressing is an attractive approach to minimize infection in a wound. In this work, copper (II) cross-linked alginate hydrogels were successfully prepared using a two-step cross-linking procedure. In the first step, solid alginate films were prepared using a solvent-casting method from soft gels of alginate solutions that had been lightly cross-linked using a copper (II) (Cu2+) sulfate solution. In the second step, the films were further cross-linked in a corresponding Cu2+ sulfate solution using a dipping method to further improve their dimensional stability. Alginate solution (at 2% w/v) and Cu2+ sulfate solution (at 2% w/v) in acetate buffer at a low pH provided soft films with excellent swelling behavior. An increase in either Cu2+ ion concentration or cross-linking time led to hydrogels with more densely-cross-linked networks that limited water absorption. The hydrogels clearly showed antibacterial activity against Escherichia coli, Staphylococcus aureus, methicillin-resistant Staphylococcus aureus (MRSA), Staphylococcus epidermidis and Streptococcus pyogenes, which was proportional to the Cu2+ ion concentration. Blood coagulation studies showed that the tested copper (II) cross-linked alginate hydrogels had a tendency to coagulate fibrin, and possibly had an effect on pro-thrombotic coagulation and platelet activation. Conclusively, the prepared films are likely candidates as antibacterial wound dressings.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available