Protein microchips: Use for immunoassay and enzymatic reactions

Different proteins such as antibodies, antigens, and enzymes were immobilized within the 100 x 100 x 20-mu m gel pads of protein microchips. A modified polyacrylamide gel has been developed to accommodate proteins of a size up to 400,000 daltons, Electrophoresis in the microchip reaction chamber speeded up antigen-antibody interactions within the gel. Protein microchips were used in immunoassays for detection of antigens or antibodies, as well as to carry out enzymatic reactions and to measure their kinetics in the absence or presence of an inhibitor. A protein microchip can be used several times in different immunoassays and enzymatic kinetic measurements. (C) 2000 Academic Press.