Journal
JOURNAL OF IMMUNOLOGY
Volume 164, Issue 4, Pages 1986-1994Publisher
AMER ASSOC IMMUNOLOGISTS
DOI: 10.4049/jimmunol.164.4.1986
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After systemic infection of mice with 10(4) PFU of lymphocytic choriomeningitis virus (LCMV), infected cells are detected simultaneously in various organs, including spleen and intestinal mucosa, Most notably, virus-infected cells are also present among CD11c(+) dendritic cells in the subepithelial area of the small intestinal mucosa, Some of these virus infected cells are in close spatial association with intestinal intraepithelial lymphocytes (IEL), Therefore, we compared virus-specific cytotoxic activity of CD8 splenocytes with that of IEL subsets. While ex vivo isolated TCR alpha beta(+)CD8 alpha alpha(+) IEL exert only minimal virus specific cytotoxicity, maximum specific killing mediated by TCR alpha beta(+)CD8 alpha beta(+) IEL on day 8 postinfection exceeds maximum cytotoxic activity observed with CD8 splenocytes when assessed in vitro. Maximum cytotoxic activity of IEL is preceded by peak perforin and granzyme B mRNA expression in IEL, around day 6 postinfection, suggesting a recent activation in situ, The antivirus cytotoxicity of in vivo primed IEL is further demonstrated by the protection from virus production in the spleen of mice infected with LCMV 10 h before adoptive cell transfer. These data indicate a potent priming of LCMV-specific IEL in situ after systemic LCMV infection and suggest that cytotoxic IEL markedly contribute to the elimination of virus-infected cells in the intestinal mucosa.
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