Journal
FREE RADICAL BIOLOGY AND MEDICINE
Volume 28, Issue 4, Pages 636-642Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/S0891-5849(99)00277-4
Keywords
melatonin; antioxidants; pesticide; phosphine; free radicals; glutathione; lipid peroxidation; 8-hydroxydeoxyguanosine
Funding
- NIEHS NIH HHS [R01 ES08762] Funding Source: Medline
Ask authors/readers for more resources
Phosphine (PH3), from hydrolysis of aluminum, magnesium and zinc phosphide, is an insecticide and rodenticide. Earlier observations on PH3-poisoned insects, mammals and a mammalian cell line led to the proposed involvement of oxidative damage in the toxic mechanism. This investigation focused on PH3-induced oxidative damage in rats and antioxidants as candidate protective agents. Male Wistar rats were treated ip with PH3 at 2 mg/kg. Thirty min later the brain, liver, and lung were analyzed for glutathione (GSH) levels and lipid peroxidation las malondialdehyde and 4-hydroxyalkenals) and brain and lung for 8-hydroxydeoxyguanosine (8-OH-dGuo) in DNA. PH3 caused a significant decrease in GSH concentration and elevation in lipid peroxidation in brain (36-42%), lung (32-38%) and liver (19-25%) and significant increase of 8-OH-dGuo in DNA of brain (70%) and liver (39%). Antioxidants administered ip 30 min before PH3 were melatonin, vitamin C, and beta-carotene at 10, 30, and 6 mg/kg, respectively. The PH3-induced changes were significantly or completely blocked by melatonin while vitamin C and beta-carotene were less effective or inactive. These findings establish that PH3 induces and melatonin protects against oxidative damage in the brain, lung and liver of rats and suggest the involvement of reactive oxygen species in the genotoxicity of PH3. (C) 2000 Elsevier Science Inc.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available