Journal
JOURNAL OF CELL BIOLOGY
Volume 211, Issue 5, Pages 945-954Publisher
ROCKEFELLER UNIV PRESS
DOI: 10.1083/jcb.201505086
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Funding
- Portuguese Foundation for Science and Technology
- FEDER/COMPETE [SFRH/BD/73532/2010]
- CRUP/Treaty of Windsor (ACCOES INTEGRAD AS) [B-33/09]
- Biotechnology and Biological Sciences Research Council
- Biotechnology and Biological Sciences Research Council [BB/J009903/1] Funding Source: researchfish
- Fundação para a Ciência e a Tecnologia [SFRH/BD/73532/2010] Funding Source: FCT
- BBSRC [BB/J009903/1] Funding Source: UKRI
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Intracellular transport is mediated by molecular motors that bind cargo to be transported along the cytoskeleton. Here, we report, for the first time, that peroxisomes (POs), lipid droplets (LDs), and the endoplasmic reticulum (ER) rely on early endosomes (EEs) for intracellular movement in a fungal model system. We show that POs undergo kinesin-3- and dynein-dependent transport along microtubules. Surprisingly, kinesin-3 does not colocalize with POs. Instead, the motor moves EEs that drag the POs through the cell. PO motility is abolished when EE motility is blocked in various mutants. Most LD and ER motility also depends on EE motility, whereas mitochondria move independently of EEs. Covisualization studies show that EE-mediated ER motility is not required for PO or LD movement, suggesting that the organelles interact with EEs independently. In the absence of EE motility, POs and LDs cluster at the growing tip, whereas ER is partially retracted to subapical regions. Collectively, our results show that moving EEs interact transiently with other organelles, thereby mediating their directed transport and distribution in the cell.
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