Journal
FEBS LETTERS
Volume 468, Issue 1, Pages 28-32Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/S0014-5793(00)01194-7
Keywords
ARGRIII; inositol polyphosphate multikinase; inositol phosphate; InsP(6); mRNA export; yeast
Funding
- NIDA NIH HHS [DA00074] Funding Source: Medline
- NIMH NIH HHS [MH18501] Funding Source: Medline
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The ARGRIII gene of Saccharomyces cerevisiae encodes a transcriptional regulator that also has inositol polyphosphate multikinase (ipmk) activity [Saiardi et al, (1999) Curr, Biol, 9, 1323-1326]. To investigate how inositol phosphates regulate gene expression, we disrupted the ARGRIII gene. This mutation impaired nuclear mRNA export, slowed cell growth, increased cellular [InsP(3)] 170-fold and decreased [InsP(6)] 100-fold, indicating reduced phosphorylation of InsP(3) to InsP(6). Levels of diphosphoinositol polyphosphates were decreased much less dramatically than was InsP(6). Low levels of InsP(6), and considerable quantities of Ins(1,3,4,5)P-4, were synthesized by an ipmk-independent route. Transcriptional control by ipmk reflects that it is a pivotal regulator of nuclear mRNA export via inositol phosphate metabolism. (C) 2000 Federation of European Biochemical Societies.
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