Journal
FEBS LETTERS
Volume 468, Issue 1, Pages 79-83Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/S0014-5793(00)01195-9
Keywords
dystroglycan; glycosylation; Chinese hamster ovary cell; dystrophin-glycoprotein complex
Funding
- NIDDK NIH HHS [DK25295] Funding Source: Medline
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Dystroglycan is a cytoskeleton-linked extracellular matrix receptor expressed in many cell types. Dystroglycan is composed of alpha- and beta-subunits which are encoded by a single mRNA. Using a heterologous mammalian expression system, we provide the first biochemical evidence of the alpha/beta-dystroglycan precursor propeptide prior to enzymatic cleavage. This 160 kDa dystroglycan propeptide is processed into alpha- and beta-dystroglycan (120 kDa and 43 kDa, respectively), We also demonstrate that the precursor propeptide is glycosylated and that blockade of asparagine-linked (N-linked) glycosylation did not prevent the cleavage of the dystroglycan precursor peptide. However, inhibition of N-linked glycosylation results in aberrant trafficking of the alpha- and beta-dystroglycan subunits to the plasma membrane. Thus, dystroglycan is synthesized as a precursor propeptide that is post-translationally cleaved and differentially glycosylated to yield alpha- and beta-dystroglycan. (C) 2000 Federation of European Biochemical Societies.
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