Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 870, Issue 1-2, Pages 245-254Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/S0021-9673(99)00863-8
Keywords
amino acids; perfluorocarboxylic acids; porous graphitic carbon; stationary phases, LC
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The analysis of twenty underivatized protein amino acids has been achieved on porous graphitic carbon packing material (Hypercarb). Five perfluoroalkyl carboxylic acids (trifluoroacetic, heptafluorobutyric, nonafluoropentanoic, tridecafluoroheptanoic and pentadecafluorooctanoic acid) have been studied as ion-pairing reagent. Several parameters (equilibration time, quantities adsorbed onto the chromatographic support, concentration and nature of the ion-pairing reagent, as well as temperature effect) have been studied leading to the complete separation of these compounds in gradient elution mode. Evaporative light scattering detector has allowed the detection of these non UV-visible absorbent molecules. The chromatographic methodology developed can also be easily coupled with pneumatically assisted electrospray mass spectrometry. (C) 2000 Elsevier Science B.V. All rights reserved.
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