Journal
JOURNAL OF IMMUNOLOGICAL METHODS
Volume 235, Issue 1-2, Pages 81-90Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/S0022-1759(99)00223-9
Keywords
rabies virus; human monoclonal antibodies; rabies post-exposure prophylaxis; passive immunization
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Funding
- NIDCR NIH HHS [N01-DE52606] Funding Source: Medline
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To provide a more defined and safer replacement for the human rabies immune globulin (HRIG) from pooled serum which is currently used for treatment of exposure to rabies virus we have developed a series of human rabies virus-specific monoclonal antibodies. Mouse-human heterohybrid myeloma cells producing rabies virus-specific human monoclonal antibodies were prepared using B cells obtained from volunteers recently-immunized with a commercial rabies virus vaccine (HDCV). Cell lines producing antibody which neutralized the Evelyn-Rokitnicki-Abelseth (ERA) rabies virus strain in vitro were cloned and the resulting monoclonal antibodies characterized for isotype, specificity against a variety of rabies virus isolates, and neutralization capacity. The ability of the monoclonal antibodies to neutralize a variety of rabies virus strains in vitro correlated with their binding specificity for these viruses in an enzyme-linked immunoadsorbant assay (ELISA), A number of these antibodies have proven suitable for the formulation of a prophylactic human monoclonal antibody-based reagent which would provide significant advantages to the HRIG in having defined, reproducible specificity, lessened possibility of contamination with viral pathogens, and consistent availability. (C) 2000 Elsevier Science B.V. All rights reserved.
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