Journal
JOURNAL OF PHYSICS-CONDENSED MATTER
Volume 12, Issue 8A, Pages A443-A449Publisher
IOP PUBLISHING LTD
DOI: 10.1088/0953-8984/12/8A/361
Keywords
-
Categories
Ask authors/readers for more resources
The salting-out effect of simple electrolytes on lysozyme has been studied by measuring the second virial coefficient B-2 Of the osmotic pressure as a function of salt concentration, and for different salts. The aim of this work has been to End a microscopic counterpart of the empirical Hofmeister series for the efficiency of cations and anions in inducing protein crystallization. The experimental results show that, for large enough ionic strengths, B-2 scales linearly with the salt concentration. This trend is common to a number of different monovalent salts, however with efficiency strongly dependent on the specific anion. Conversely, changing the cation does not appreciably affect B-2. The significance of these findings for the investigation-of protein interactions near crystallization is discussed.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available