Journal
JOURNAL OF CHROMATOGRAPHY B
Volume 739, Issue 1, Pages 219-224Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/S0378-4347(99)00469-7
Keywords
riboflavin; flavin nucleotides; vitamers
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We describe an assay which determines simultaneously riboflavin (RF), flavin mononucleotide (FMN) and flavin adenine dinucleotide (FAD) in plasma, using galactoflavin (GF) as an internal standard. The flavins were extracted on a C-18 Sep-Pack cartridge after protein precipitation with 10% trichloroacetic acid, and were analyzed on a C-18 RP-HPLC with 85% phosphate-magnesium acetate buffer (pH 3.4) and 15% acetonitrile. FAD, FMN, GF and RF extraction recoveries were 101.0+/-5.6, 97.0+/-6.5, 97.0+/-2.0 and 95.0+/-4.1%, and reproducibilities were 5.9, 6.8, 2.1 and 4.3%, respectively. FAD, FMN and RF values in infant and adolescent plasma were in the range 53.5-108.2, 9.0-25.1 and 12.7-53.4 nM, and 36.5-157.20, 7.1-24.6 and 8.2-57.8 nM, respectively. Using GF as an internal standard improved the quantification of these B2 vitamers. (C) 2000 Elsevier Science BN. All rights reserved.
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