Journal
PROTEIN EXPRESSION AND PURIFICATION
Volume 18, Issue 2, Pages 141-147Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1006/prep.1999.1126
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Laccase from Rigidoporus lignosus, a white-rot basidiomycete, has been isolated from culture filtrates. The enzyme was purified to homogeneity and some of its structural and kinetic parameters have been determined. The effects of pH, temperature, and organic solvents on the activity and stability of the enzyme, under different conditions, were also assayed. The results we have obtained, including the rather broad substrate specificity of enzyme, combined with their relatively easy production and purification, suggest that laccase may be efficiently employed in a variety of biotechnology applications. (C) 2000 Academic Press.
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