HPLC-ELSD determination of kanamycin B in the presence of kanamycin A in fermentation broth

A novel method for the direct determination of kanamycin B in the presence of kanamycin A in fermentation broth using high performance liquid chromatography with evaporative light scattering detector (HPLC-ELSD) was developed. An Agilent Technologies C-18 column was utilized, evaporation temperature of 40 degrees C and nitrogen pressure of 3.5bar, the optimized mobile phase was water-acetonitrile (65:35, v/v), containing 11.6mm heptafluorobutyric acid (isocratic elution with flow rate of 0.5mL/min) with the gain 11. Kanamycin B was eluted at 5.6min with an asymmetry factor of 1.827. The method showed good linearity over the concentration range of 0.05 to 0.80mg/mL for the kanamycin B (r(2)=0.9987). The intra-day and inter-day coefficients of variation obtained from kanamycin B were less than 4.3%. Mean recovery of kanamycin B from spiked fermentation broth was 95%. The developed method was applied to the determination of kanamycin B without any interference from other constituents in the fermentation broth. This method offers simple, rapid and quantitative detection of kanamycin B. Copyright (c) 2014 John Wiley & Sons, Ltd.