Journal
BIOMEDICAL CHROMATOGRAPHY
Volume 29, Issue 2, Pages 312-320Publisher
WILEY
DOI: 10.1002/bmc.3277
Keywords
magnetic nanoparticles; extraction capacity; cis-diol-containing compounds; high-performance liquid chromatography
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Funding
- National Natural Science Foundation of China [21275115, 20975080]
- Chinese Ministry of Education [212178]
- NWU Undergraduate Innovative Experimental Research Programs [2013043]
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The use of bronate affinity adsorbents is a new separation method that appeared recently with great potential for specific extraction of cis-diol-containing compounds. In this work,a new strategy for the facile construction of boronic acid-functionalized Fe3O4 magnetic nanoparticles (Fe3O4@FPBA MNPs) with a high capacity was described. The extraction capacity of the Fe3O4@FPBA MNPs was determined to be 66.0 +/- 2.7 mu mol/g for catechol and 80.6 +/- 2.0 mu mol/g for dopamine, being higher than that for the reported methods. The Fe3O4@FPBA MNPs were used to extract four cis-diol drugs: caffeic acid isopropyl ester, caffic acid bornyl ester, isopropyl 3-(3,4-dihydroxyphenyl)-2-hydroxypropanoate and 3-(3, 4-dihydroxyphenyl)-2-hydroxylpropionic acid - from the spiked rabbit plasma, and the recoveries of four drugs were between 87.29 and104.37% with relative standard deviations ranging from 1.34 to 8.81%. Under the most favorable conditions, the solid-phase extraction combined with HPLC-UV for the analysis of four drugs in plasma could eliminate interferences from endogenous components of the biological fluids and exhibited sufficient precision and accuracy. These results showed that the prepared Fe3O4@FPBA MNPs were qualified for efficiently enriching and determining the trace cis-diol substances from biological samples. Copyright (c) 2014 John Wiley & Sons, Ltd.
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