Journal
MECHANISMS OF DEVELOPMENT
Volume 91, Issue 1-2, Pages 61-68Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/S0925-4773(99)00271-3
Keywords
alkaline phosphatase; allantois; cell migration; filopodia; FM4-64; germline; green fluorescent protein; hind-gut; mouse-murine development; Oct4; primitive streak; primordial germ cell; SSEA-I; time-lapse confocal microscopy
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Funding
- NICHD NIH HHS [HD33440-01] Funding Source: Medline
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Mouse primordial germ cells (PGCs) are specified between embryonic day 6.5 (E6.5) and E7.5, when they have been visualized as an alkaline phosphatase-positive (AP+) cell population in the developing allantois. By E8.5, they are embedded in the hind-gut epithelium. Previous experiments have suggested different sites for PGCs' origin, and it is unclear how they reach the gut epithelium. We have used transgenic mice expressing GFP under a truncated Oct4 promoter to visualize living PGCs. We find GFP+/AP+ cells in the posterior end of the primitive streak as a dispersed population of cells actively migrating into the allantois, and directly into the adjacent embryonic endoderm. Time-lapse analysis shows these cells to be actively migratory from the time they exit the primitive streak. (C) 2000 Elsevier Science Ireland Ltd. All rights reserved.
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