Journal
BIOMEDICAL CHROMATOGRAPHY
Volume 27, Issue 7, Pages 889-893Publisher
WILEY-BLACKWELL
DOI: 10.1002/bmc.2877
Keywords
temozolomide; LC-MS; MS; pharmacokinetics; mice; brain distribution
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A sensitive and accurate liquid chromatography method with mass spectrometry detection was developed and validated for the quantification of temozolomide in mouse plasma and brain. Theophyllin was used as the internal standard. A single-step protein precipitation was used for plasma and brain sample preparation. The method was validated with respect to selectivity, extraction recovery, linearity, intra- and inter-day precision and accuracy, limit of quantification and stability. The method has a limit of quantification of 50ng/mL for temozolomide in plasma and 125ng/g in brain. This method was used successfully to perform brain and plasma pharmacokinetic studies of temozolomide in mice after intraperitoneal administration. Copyright (c) 2013 John Wiley & Sons, Ltd.
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