Journal
BIOMEDICAL CHROMATOGRAPHY
Volume 28, Issue 3, Pages 327-331Publisher
WILEY-BLACKWELL
DOI: 10.1002/bmc.3056
Keywords
red blood cells; reduced and total aminothiols; redox state; HPLC
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- Driatec, Cassina de' Pecchi, Milan, Italy
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Detection and quantification of different aminothiols forms (reduced and total) in biological fluids are important for the investigation of oxidative stress-related diseases and cell homeostasis study. The aim of this study was to optimize a HPLC method in order to determine both reduced and total thiol forms in red blood cells (RBC) at low temperature without washing erythrocytes. Analytical recoveries for total and reduced thiols were 91.6-98.5 and 94.9-98.2% respectively. The relative standard deviations intra-assay for total and reduced thiols were 1.14-3.64 and 0.83-2.3% respectively and the relative standard deviations inter-assay for total and reduced thiols were 1.12-3.54 and 0.84-2.03%, respectively. This method allows specific analysis of the aminothiol state inside the RBC, as a model of intracellular metabolism functioning. Copyright (c) 2013 John Wiley & Sons, Ltd.
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