Journal
BIOMEDICAL CHROMATOGRAPHY
Volume 27, Issue 9, Pages 1123-1128Publisher
WILEY
DOI: 10.1002/bmc.2916
Keywords
arctigenin; LC/MS/MS; pharmacokinetics; rat plasma
Categories
Funding
- national 973 program of China [2010CB933900]
- national science and technology major projects of China [2012ZX09304002]
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In this study, a simple and sensitive LC/MS/MS method was developed and validated for the determination of arctigenin in rat plasma. The MS detection was performed using multiple reaction monitoring at the transitions of m/z 373.2 -> 137.3 for arctigenin and m/z 187.1 -> 131.0 for psoralen (internal standard) with a Turbo IonSpray electrospray in positive mode. The calibration curves fitted a good linear relationship over the concentration range of 0.2-500 ng/mL. It was found that arctigenin is not stable enough at both room temperature and -80 degrees C unless mixed with methanol before storage. The validated LC/MS/MS method was successfully applied for the pharmacokinetic study of arctigenin in rats. After intravenous injection of 0.3 mg/kg arctigenin injection to rats, the maximum concentration, half-life and area under the concentration-time curve were 323 +/- 65.2ng/mL, 0.830 +/- 0.166 and 81.0 +/- 22.1 h ng/mL, respectively. Copyright (C) 2013 John Wiley & Sons, Ltd.
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