Journal
JOURNAL OF CELL BIOLOGY
Volume 211, Issue 3, Pages 533-551Publisher
ROCKEFELLER UNIV PRESS
DOI: 10.1083/jcb.201504035
Keywords
-
Categories
Funding
- Italian Ministry of Education, University, and Research [RBFR106S1Z_001]
- flagship Consiglio Nazionale delle Ricerche project (Epigen)
Ask authors/readers for more resources
Beyond its role in providing structure to the nuclear envelope, lamin A/C is involved in transcriptional regulation. However, its cross talk with epigenetic factors and how this cross talk influences physiological processes is still unexplored. Key epigenetic regulators of development and differentiation are the Polycomb group (PcG) of proteins, organized in the nucleus as microscopically visible foci. Here, we show that lamin A/C is evolutionarily required for correct PcG protein nuclear compartmentalization. Confocal microscopy supported by new algorithms for image analysis reveals that tannin A/C knock-down leads to PcG protein foci disassembly and PcG protein dispersion. This causes detachment from chromatin and defects in PcG protein mediated higher-order structures, thereby leading to impaired PcG protein repressive functions. Using myogenic differentiation as a model, we found that reduced levels of lamin A/C at the onset of differentiation led to an anticipation of the myogenic program because of an alteration of PcG protein mediated transcriptional repression. Collectively, our results indicate that lamin A/C can modulate transcription through the regulation of PcG protein epigenetic factors.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available