Journal
BIOMEDICAL CHROMATOGRAPHY
Volume 26, Issue 4, Pages 464-470Publisher
WILEY
DOI: 10.1002/bmc.1688
Keywords
citrus; flavonoids; coumarin; HPLC-MS; MS; antioxidant activity
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Funding
- National Research Foundation of Korea
- Korean government (MEST) [2009-0084454]
- National Research Foundation of Korea [2009-0084454] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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A mixture of flavonoid components was isolated from the fruit peel of three varieties of citrus native to Republic of Korea, Citrus leiocarpa Hort. ex Tanaka (CLHT), Citrus aurantium L. (CAL) and Citrus erythrosa Hort. (CEH), via 70% methanol extraction followed by ethyl acetate elution over a silica gel cartridge. The flavonoid components of the mixture were analyzed via high-performance liquid chromatographytandem mass spectrometry (HPLC-MS/MS) in positive-ion mode and a comparison of the reported data. Among 17 characterized components, two flavanones, four flavones and two coumarin derivatives in the fruit peel of the three varieties were identified for the first time. The individual characterized components were quantified via HPLC-UV. The flavanones dominated in CAL, whereas the flavones prevailed in CLHT and CEH. The antioxidant activity of the flavonoid mixture of the fruit peel was determined via DPPH, ABTS+ and reducing power assays. The antioxidant activity of CEH and CAL was greater than that of CLHT. Copyright (c) 2011 John Wiley & Sons, Ltd.
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