Journal
BIOMEDICAL CHROMATOGRAPHY
Volume 25, Issue 3, Pages 417-426Publisher
WILEY
DOI: 10.1002/bmc.1467
Keywords
anthraquinone glycosides; intestinal bacteria; metabolites; LC-DAD; LC-ESI-MS/MS
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Funding
- Specialized Research Fund for TCM of State Administration of Traditional Chinese Medicine [06-07ZP17]
- Chinese Natural Science Fund [30672587]
- Technology Innovation Program for Post-graduates of Jiangsu Province [CX08B_194Z]
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Through investigation of the metabolism of rhubarb extract by rat intestinal bacteria, a total of 14 components in rhubarb extract were found to be biotransformed. These components included aloe-emodin-O-glucosides, emodin-O-glucosides, chrysophanol-O-glucosides, physcion-O-glucosides and the corresponding aglycones. Rhein also could be biotransformed by rat intestinal bacteria. Twelve major metabolites were detected in the incubation sample. Under ESI tandem mass conditions, the sequential fragmentation patterns of [M - H](-) ions were similar to those of free anthraquinones, thus allowing the rapid identification of the metabolites formed in incubation samples. The results suggested that the proposed hydrolysis of glycoside group followed by hydrogenation in quinoid moiety and/or further acetylation was the major biotransformation pathway for these anthraquinone glycosides by rat intestinal bacteria. Copyright (C) 2010 John Wiley & Sons, Ltd.
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