Journal
BIOMEDICAL CHROMATOGRAPHY
Volume 26, Issue 5, Pages 650-654Publisher
WILEY-BLACKWELL
DOI: 10.1002/bmc.1710
Keywords
2-deoxyglucose; HPLC; fluorescence derivatization; pharmacokinetics
Categories
Funding
- Department of Defense [W81XW8-05-1-0036]
- Cancer Institute of New Jersey [P30CA072720]
Ask authors/readers for more resources
2-Deoxyglucose (2-DG), an analog of glucose, is widely used to interfere with glycolysis in tumor cells and studied as a therapeutic approach in clinical trials. To evaluate the pharmacokinetics of 2-DG, we describe the development and validation of a sensitive HPLC fluorescent method for the quantitation of 2-DG in plasma. Plasma samples were deproteinized with methanol and the supernatant was dried at 45 degrees C. The residues were dissolved in methanolic sodium acetateboric acid solution. 2-DG and other monosaccharides were derivatized to 2-aminobenzoic acid derivatives in a single step in the presence of sodium cyanoborohydride at 80 degrees C for 45 min. The analytes were separated on a YMC ODS C18 reversed-phase column using gradient elution. The excitation and emission wavelengths were set at 360 and 425 nm. The 2-DG calibration curves were linear over the range of 0.63-300 mu g/mL with a limit of detection of 0.5 mu g/mL. The assay provided satisfactory intra-day and inter-day precision with RSD less than 9.8%, and the accuracy ranged from 86.8 to 110.0%. The HPLC method is reproducible and suitable for the quantitation of 2-DG in plasma. The method was successfully applied to characterize the pharmacokinetics profile of 2-DG in patients with advanced solid tumors. Copyright (c) 2011 John Wiley & Sons, Ltd.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available