Journal
BIOMEDICAL CHROMATOGRAPHY
Volume 26, Issue 3, Pages 350-354Publisher
WILEY
DOI: 10.1002/bmc.1664
Keywords
cisplatin; telomere; DNA damage; DNA sequence selectivity
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Funding
- University of NSW, Science Faculty
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The determination of the sequence selectivity of DNA-damaging agents is very important in elucidating the mechanism of action of anti-tumour drugs. The development of automated capillary DNA sequencers with fluorescent labelling has enabled a more precise method for DNA sequence specificity analysis. In this work we utilized the ABI 3730 capillary sequencer with laser-induced fluorescence to examine the sequence selectivity of cisplatin with purified DNA sequences. The use of this automated machine enabled a higher degree of precision of both position and intensity of cisplatinDNA adducts than previously possible with manual and automated slab gel procedures. A problem with artefact bands was overcome by ethanol precipitation. It was found that cisplatin strongly formed adducts with telomeric DNA sequences. Copyright (C) 2011 John Wiley & Sons, Ltd.
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