Journal
BIOMEDICAL CHROMATOGRAPHY
Volume 25, Issue 8, Pages 938-942Publisher
WILEY
DOI: 10.1002/bmc.1550
Keywords
kynurenine; tryptophan; high-performance liquid chromatography; electrochemical detection; chemically modified electrodes
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Funding
- National Scientific Foundation of China [20775055, 30973672, 90817103]
- Important National Science and Technology Specific Projects [2009ZX09301-14]
- Fundamental Research Funds for the Central Universities
- Wuhan University [306276216, 306271159]
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A novel method was developed for the simultaneous determination of kynurenine and tryptophan by high-performance liquid chromatography with electrochemical detection at multi-wall carbon nanotube (MWCNT)-modified glassy carbon electrode. The separation and detection conditions were optimized. The typical HPLC experiments were conducted by using a reversed-phase ODS column with a mobile phase consisting of stock acetate buffer (pH 5)-methanol (4:1, v/v) using an isocratic elution at the flow rate of 1.0 mL/min. The obtained LODs for kynurenine and tryptophane were 0.5 and 0.4 mu mol/L, respectively. The analytical method for human plasma samples was validated and confirmed by LC-UV and LC-MS. The recoveries were in the range of 84.8-110%, and the precision was lower than 5.9%. Copyright (C) 2010 John Wiley & Sons, Ltd.
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