Journal
SCIENCE
Volume 287, Issue 5458, Pages 1641-1644Publisher
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.287.5458.1641
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- NIGMS NIH HHS [GM41333, GM32441] Funding Source: Medline
- Telethon [971] Funding Source: Medline
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The Large chlorella virus PBCV-1, which contains double-stranded DNA (dsDNA), encodes a 94-codon open reading frame (ORF) that contains a motif resembling the signature sequence of the pore domain of potassium channel proteins. Phylogenetic analyses of the encoded protein, Kcv, indicate a previously unidentified type of potassium channel. The messenger RNA encoded by the ORF Leads to functional expression of a potassium-selective conductance in Xenopus laevis oocytes. The channel blockers amantadine and barium, but not cesium, inhibit this conductance, in addition to virus plaque formation. Thus, PBCV-1 encodes the first known viral protein that functions as a potassium-selective channel and is essential in the virus Life cycle.
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