Journal
SCIENCE
Volume 287, Issue 5458, Pages 1639-1640Publisher
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.287.5458.1639
Keywords
-
Categories
Funding
- NIAID NIH HHS [R29AI40634] Funding Source: Medline
- NIDDK NIH HHS [R01DK44842] Funding Source: Medline
Ask authors/readers for more resources
The type of RNA editing found in the kinetoplast-mitochondria of trypanosomes and related protozoa, involving uridylate insertions and deletions, creates translatable messenger RNAs (mRNAs) out of nonsense pre-edited RNAs by correcting encoded defects that vary from simple frameshifts to Large cryptic regions. However, any evidence for translation of these mRNAs in the kinetoplast has been missing for decades. We identified a kinetoplast-encoded protein, apocytochrome b, whose mRNA is edited in the 5' region. The determined amino-terminal sequence of the protein coincides with the predicted sequence derived from the edited region, demonstrating that the cognate apocytochrome b mRNA is translated into a functional protein. This finding represents the first direct evidence for a functional translation system in the kinetoplasts.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available